Article Abstract:
Former primer 63f and reverse primer 1387r were designed to amplify approximately 1,300 bp of a consensus 16S rRNA gene from bacteria. The efficacy and specificity of these two PCR primers were tested by using them to amplify 16 rRNA genes from template DNA taken from a wide range of organisms, including Eubacterium species and organisms from the coryneform and Micrococcus genera. The amplimer pair 63f-1387r was found to be more successful than the amplimer pair 27f-1392r in amplifying the 16S rRNA genes.
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Article Abstract:
The authors describe the study on dehalogenase gene pool in bacteria isolated on dichloropropionic acid. They have investigated the relationship between this gene pool and environmetal metagene pool from which bacteria have been isolated via the use of culture-independent and culture-dependent approaches, and the results demonstrate the existence of a large bias introduced by culturing both in isolated bacteria and in the degradative genes from these bacteria.
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Article Abstract:
Lateral gene transfer (LGT) plays a vital role in increasing the genetic diversity of microorganisms and promoting the spread of fitness-enhancing phenotypes throughout microbial communities. A study is presented in which phylogenetic analyses is used to examine the role of LGT in the evolution of metal homeostasis in subsurface bacteria.
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