Design and application of highly responsive fluorescence resonance energy transfer biosensors for detection of sugar in living Saccharomyces cerevisiae cells

Article Abstract:

Combinatorial engineering of the domain linker and the binding protein moiety are used for developing a protein sensor with a highly responsive fluorescence resonance energy transfer (FRET) signal for sensing sugars in living Saccharomyces cerevisiae cells. Highly responsive FRET images are generated from real-time analysis of maltose uptake of Saccharomyces cerevisiae when optimized maltose sensors are used as in vivo sensors.

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Secretion of recombinant pediocin PA-1 by Bifidobacterium longum, using the signal sequence for Bifidobacterial alpha-amylase

Article Abstract:

A recombinant DNA, encoding the chimeric protein of the signal sequence for bifidobacterial alpha-amylase mature pediocin PA-1, was introduced into Bifidobacterium longum MG1. Biologically active pediocin PA-1 was successfully secreted from the strain and showed bactericidal activity against Listeria monocytogenes and the same molecular mass as native pediocin PA-1.

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Efficient library construction by in vivo recombination with a telomere-originated autonomously replicating sequence of Hansenula polymorpha

Article Abstract:

Research has been conducted on Hansenula polymorpha recombinant gene expression system. The authors describe in vivo recombination techniques developed for the mutant library construction with equal gene expression level.

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