Deletions in the carboxyl-terminal region of Streptococcus gordonii glucosyltransferase affect cell-associated enzyme activity and sucrose-associated accumulation of growing cells

Article Abstract:

The CH2RPE and CH4RPE insertional mutant strains of Streptococcus gordonii Challis CH1 were utilized to determine the effects of mutations on the activity of the cell-associated glucosyltransferase (GTF). Changes in the carboxy-terminal region of GTF decreased enzyme activity without altering the levels of GTF proteins in the mutant strains. Insertional deletion of the 14 carboxy-terminal amino acids of GTF in CH2RPE and CH4RPE reduced by the levels of cell-associated GFT proteins due to reduced sucrose-associated accumulation.

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Effects of ionic and osmotic strength on the glucosyltransferase of Rhizobium meliloti responsible for cyclic beta-(1,2)-glucan biosynthesis

Article Abstract:

Research was conducted to investigate the effects of ionic and osmotic strength on the glucosyltransferase of Rhizobium meliloti responsible for cyclic beta-(1,2)-glucan biosynthesis. The regulation of the biosynthesis of these glucans were examine in vitro with membrane preparations from R meliloti. Results indicate that NdvA and the C terminus of NdvB are not essential for osmotic regulation of cyclic beta-(1,2)-glucan biosynthesis.

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Mutagenesis of Asp-569 of glucosyltransferase I glucansucrase modulates glucan and oligosaccharide synthesis

Article Abstract:

Asp-569 of glucosyltransferase I glucansucrase modulates glucan and oligosaccharide synthesis by its mutagenesis. Engineering the equivalent position in glucansucrases is potentially a way to increase the range of oligosaccharides synthesized.

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