Degradation of polychlorinated biphenyl metabolites by naphthalene-catabolizing enzymes

Article Abstract:

A study was conducted to examine the capability of the dehydrogenase and ring cleavage dioxygenase of the naphthalene degradation pathway to change 3,4-dihydroxylated biphenyl metabolites. Escherichia coli M15(pREP4) and SG13009 were utilized as bacterial strains while protein characterization was carried out using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results indicated that the recruitment of catabolic enzymes supporting specificities supporting enzymes of the biphenyl degradation pathway may lead to the degradation of polychlorinated biphenyls.

author: Eltis, Lindsay D., Barriault, Diane, Durand, Jacinthe, Maaroufi, Halim, Sylvestre, Michel
Enzymes, Dehydrogenases, Oxidoreductases, Naphthalene, Biphenyl compounds, Biphenyls (Class of compounds)

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Catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments

Article Abstract:

The catechol 2,3-dioxygenase kinetic parameters of Pseudomonas sp. strain W31, Pseudomonas picketti PKO1 and Pseudomonas fluorescens CFS215 from hypoxic environments are compared with those of three archetypal aerobic BTEX degraders. Results that the three bacterial strains have the ability to carry out significant nitrate-dependent degradation of aromatic hydrocarbons in oxygen-limited conditions.

author: Kukor, Jerome J., Olsen, Ronald H.
Pseudomonas, Catecholamine metabolism

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Subculturing of a polychlorinated biphenyl-dechlorinating anaerobic enrichment on solid media

Article Abstract:

Polychlorinated biphenyls (PCB)-dechlorinating anaerobes were subcultured on solid media to isolate an anaerobic PCB-dechlorinating organism. A combination of anaerobic enrichment procedures were used in the dechlorination activity and on the transfers on solid medium. It was found that PCB-dechlorination was caused by anaerobic bacteria and can be grown on solid media even without PCB.

author: May, Harold D., Boyle, Alfred W., Price, W. Allen, II, Blake, Cheryl K.

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subjects list: Research, Polychlorinated biphenyls, Analysis, Anaerobic bacteria
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