Degradation of phthalate and di-(2-ethylhexyl)phthalate by indigenous and inoculated microorganisms in sludge-amended soil

Article Abstract:

A study was conducted to analyze the microbial degradation of di(2-ethylhexyl)phthalate (DEHP) and the degradation intermediate phthalic acid found in agricultural soil supporting sewage sludge. 14C-labelled substrates and various isotope methods were utilized to determine the transformation of DEHP and phthalic acid. Results indicated that DEHP and phthalic acid can be changed to different intermediates at a rate that may surpass mineralization rates. Findings also showed the the bioavailability of substrates correlated with the enzymatic degradation of hydrophobic organic pollutants.

author: Roslev, Peter, Henriksen, Kaj, Madsen, Peter L., Thyme, Jesper B.
Phthalate esters, Phthalate plasticizers, Soil research, Sewage sludge

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Oxidation and assimilation of atmospheric methane by soil methane oxidizers

Article Abstract:

Methane assimilation/oxidation by forest soil microorganisms and endogenous carbon turnover was measured by radiotracer techniques during changes in temperature, methane/ammonia concentration and soil structure. The capacity of bacterial growth on atmospheric methane does not occur in a microbial consortium. Furthermore, soil methane metabolism by methane- and ammonia-oxidizing bacteria was affected by atmospheric methane concentration which is used as a true source of cell carbon by soil bacteria.

author: Roslev, Peter, Henriksen, Kaj, Iversen, Niels
Physiological aspects, Microbial metabolism, Microbiological synthesis, Soil microbiology, Methanobacteriaceae, Methanogens

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Radioactive fingerprinting of microorganisms that oxidize atmospheric methane in different soils

Article Abstract:

Research was conducted to examine the radioactive fingerprinting of microorganisms that oxidize atmospheric methane in different soils. The methane metabolism and the diversity of the organisms that oxidized atmospheric methane in soil samples from Arctic, temperate and tropical regions were compared. An analysis of radiolabelled phospholipid ester-linked fatty acids provided a radioactive fingerprint of the active soil methanotrophs.

author: Roslev, Peter, Iversen, Niels
Methods, Usage, Genetic aspects, Methane, DNA testing, DNA identification, Microorganisms, Radioactive tracers

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subjects list: Research, Analysis
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