Degradation of Giardia lamblia cysts in mixed human and swine wastes

Article Abstract:

The degradation of Giardia lamblia cysts in mixed septic tank effluent (STE) and swine manure slurry (SMS) was found to be faster than in pure STE and in the saline control. Effectiveness of degradation increased with the proportion of SMS, hence SMS is thought to contain the degrading agent. Cysts were degraded more rapidly at higher incubation temperatures, although freezing temperatures do kill the cysts. Staining with fluorescein diacetate-propidium iodide proved to be a reliable and convenient method of assessing viability since results correlated well with electron microscopic findings.

author: Deng, Ming Yi, Cliver, Dean O.
Organic wastes

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Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples

Article Abstract:

The selective concentration of Giardia cycsts from water samples by an antibody-magnetite concentration method is discussed. This involved the utilization a primary antibody and a secondary labeling reagent. High-gradient magnetic separation was used to concentrate the magnetically-labeled cysts. The potential of the method for linkage with indirect fluorescent-antibody detection methods was demonstrated.

author: Bifulco, Joseph M., Schaefer, F.W., III
Usage, Magnetite, Immunoenzyme technique, Immunoenzyme techniques

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Comparison of levels of inactivation of two isolates of Giardia lamblia cysts by UV light

Article Abstract:

A collimated beam protocol and a Mongolian gebril model are used to assess the effects of ultraviolet (UV) irradiation on two human isolates (WB and H3) of Giardia lamblia cysts. The results have shown that the gerbils are more susceptible to isolate WB cysts after the cysts are exposed to UV light.

author: Belosevic, Miodrag, Dong Li, Craik, Stephen A., Smith, Daniel W.
Science & research, Environmental aspects, Ultraviolet radiation

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subjects list: Research, Giardia, Giardia lamblia
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