Degradation of 1,2,3,4-tetrachlorobenzene by Pseudomonas chlororaphis RW71

Article Abstract:

The aerobic mineralization of 1,2,3,4-tetrachlorobenzene by a pure culture of Pseudomonas chlororaphis RW71 was demonstrated. The reaction produced stoichiometric amounts of chloride. The transient excretion of tetrachlorocatechol in the early growth phase suggested an initial attack by a dioxygenase to form the corresponding dihydrodiol which rearomatizes to the catechol. Extraordinarily high activity was found in chlorocatechol 1,2-dioxygenase in crude cell extracts.

author: Wittich, Rolf-Michael, Timmis, Kenneth Nigel, Potrawfke, Thomas
Pseudomonas, Biomineralization, Chlorobenzene, Chlorobenzenes

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Degradation of chlorinated dibenzofurans and dibenzo-p-dioxins by Sphingomonas sp. strain RW1

Article Abstract:

The bacterium Sphingomonas sp. strain RW1 degrades many mono- and dichlorinated dibenzofurans, and dibenzo-p-dioxins, to the mono- and dichlorinated salicylates and catechols. The degradation is apparently a dioxygenolytic attack on the substituted and nonsubstituted aromatic nucleus of the target compounds. The strain RW1 uses monochlorinated dibenzo-p-dioxins and dibenzofurans as the carbon source.

author: Wittich, Rolf-Michael, Francke, Wittko, Timmis, Kenneth N., Wilkes, Heinz, Fortnagel, Peter
Analysis, Bacteria, Dibenzofurans

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Chromosomal integration of tcb chlorocatechol degradation pathway genes as a means of expanding the growth substrate range of bacteria to include haloaromatics

Article Abstract:

Researchers transferred a gene cluster for the chlorocatechol ortho-cleavage pathway into Pseudomonas putida KT2442. This research is based on efforts to genetically modify bacteria so they can biodegrade haloaromatic compounds.

author: Klemba, Michael, Jakobs, Barbara, Wittich, Rolf-Michael, Pieper, Dietmar
Microbial genetic engineering

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subjects list: Research, Biodegradation
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