Article Abstract:
New strains of Ancylobacter aquaticus capable of growing on 1,2-dichloroethane (DCE) as the sole carbon and energy source were isolated. The three strains, designated as AD20, AD25 and AD27, were facultative methylotrophs capable of utilizing DCE, 2-chloroethanol, chloroacetate and 2-chloropropionate. Studies on DCE degradation showed that the three strains and three other newly described strains of DCE degraders, followed the same pathway of DCE degradation, but differed in the regulation ofthe enzymes involved. Sequence analysis of dhlA, which codes for haloalkane dehalogenase showed that the dhlA coding regions are conserved among different species.
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Article Abstract:
The kinetic properties of Xanthobacter autotrophicus GJ10, Ancylobacter aquaticus AD20 and Pseudomonas sp. strain AD1 were measured during growth in continuous cultures and compared to the kinetic properties of the first catabolic enzyme of halogenated aliphatic compound degradation. The results showed that the strains followed Monod kinetics. Correlations between the Monod constants and Km values of the first catabolic enzyme were also observed.
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Article Abstract:
The transformation of 1,2,4-trichlorobenzene (TCB) by Burkholderia sp. strain PS14 begins at a first-order relationship between transformation rate and substrate concentration at low concentrations of 1,2,4-TCB. As substrate concentration increases, the reaction shifts to a second-order relationship.
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