DNA recovery from soils of diverse composition

Article Abstract:

The sodium dodecyl sulfate (SDS) method of bacterial DNA extraction from soils is simple, quick and efficient. The method involves bacterial lysis with a high-salt extraction buffer, followed by the heating of the soil suspension with SDS, hexadecyltrimethylammonium bromide and proteinase K. A combination of SDS with grinding and freezing-thawing increases the DNA extraction efficiency. The best DNA purification method is the gel-plus-minicolumn method. The gel-plus-concentration method gives the highest DNA recovery rate.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Simultaneous recovery of RNA and DNA from soils and sediments

Article Abstract:

Researchers modified a grinding-based cell lysis method to extract both RNA and DNA from soil samples and found that anion exchange resin produced the purest yield of RNA. The nucleic acids recovered using this method are pure enough for nuclease digestion, microarray hybridization, and PCR or reverse transcription-PCR amplification.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Molecular diversity of sulfate-reducing bacteria from two different continental margin habitats

Article Abstract:

Results point out that sulfate-reducing bacteria from continental margin sedimentary habitats display extensive genetic diversity as shown by restriction fragment length polymorphism analysis of 1,762 clones. Data indicate that the biodiversity is associated with slope depth determining carbon bioavailability.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: