Cytogenetic analysis of the Tamaraw (Bubalus mindorensis): A comparison of R-banded karyotype and chromosomal distribution of centromeric satellite DNAs, telomeric sequence, and 18S-28S rRNA genes with domestic water buffaloes

Article Abstract:

The karyotype of the tamaraw was studied using RBG-banding methods and compared with those of the river and swamp cytotypes of domestic water buffalo. The tamaraw karyotype comprises six submetacentric and 16 acrocentric autosome pairs and X and Y chromosome. Chromosome 2 of the tamaraw had larger hybridization signals for the buffalo satellite 1 DNA, and it may have occurred due to relatively recent centromeric fusion.

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Chromosomal mapping of 18S-28S rRNA genes and 10 cDNA clones of human chromosome 1 in the Musk Shrew

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18S-28S ribosomal RNA genes and 10 human cDNA clones on musk shrew chromosomes were map using direct R-banding fluorescence in situ hybridization.

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Characterization and chromosomal distribution of satellite DNA sequences of the Water Buffalo

Article Abstract:

Two types of satellite DNA sequences from the swamp-type buffalo and river-type buffalo were molecularly cloned and characterized, and the chromosomal distribution of the satellite DNA sequences was investigated, using fluorescence in situ hybridization (FISH). Centromere-specific satellite DNA was found in the interstitial region of the long arm of chromosome 1 of the swamp-type buffalo. The intensity of the hybridization signals with buffalo satellite II DNA included the Y chromosome.

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