Cytochrome c550 expression in Paracoccus denitrificans strongly depends on growth condition: identification of promoter region for cycA by transcription start analysis

Article Abstract:

Cells of Paracoccus denitrificans grown under anaerobic, denitrifying conditions or methylotropically in the presence of oxygen contained much more periplasmic cytochrome c550 than cells grown aerobically on multicarbon substrates. A similar pattern was seen when expression of cycA gene was examined using an Escherichia coli alkaline phosphatase gene fusion as a reporter of cycA promoter activity. Findings indicated that expression of cycA is affected by multiple regulatory controls and that cytochrome c550 has a general function in electron transfer to periplasmic reductases needed for anaerobic denitrifying growth and from dehydrogenases needed for aerobic growth on C1 compounds.

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The Paracoccus denitrificans ccmA, B and C genes: cloning and sequencing, and analysis of the potential of their products to form a haem or apo- c-type cytochrome transporter

Article Abstract:

The cloning and sequencing of Paracoccus denitrificans ccmABC gene region and the isolation of its mutants were reported. The c-type cytochrome deficient mutants of P. denitrificans , HN49 and HN53, were obtained by chemical mutagenesis. They fail to accumulate apocytochrome c sub-550 to the levels observed in other mutants. The three genes designated as ccmA, ccmB and ccmC were observed to form a complex with organization and they transport neither c-type apocytochromes nor haem.

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Identification of the contiguous Paracoccus denitrificans ccmF and ccmH genes: disruption of ccmF, encoding a putative transporter, results in formation of an unstable apocytochrome c and deficiency in siderophore production

Article Abstract:

Apocytochrome c550 was found in the periplasm of a new mutant of Paracoccus denitrificans, HN48, that does not have c-type cytochromes, has decreased amounts of siderophores, and has a Tn5 insertion in the ccmF gene. A counterpart to the ccmF gene was observed in an archaebacterium but could not be found in the yeast genome, while the mitochondrial heme lyases in the latter were not found in an archaeobacterial or in eubacterial genomes.

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