Culture isolation and culture-independent clone libraries reveal new marine Synechococcus ecotypes with distinctive light and N physiologies

Article Abstract:

The role that multiple ecotypes play in the ecological success of the genus marine Synechococcus during their annual peak in abundance by simultaneously using culture isolation and environmental sequence clone libraries to assess diversity is studied. Clonal isolates are obtained by enrichment with ammonium, nitrite, or nitrate as the methods revealed a total of seven Synechococcus phylotypes including two new putative ecotypes, labeled XV and XVI.

author: Rocap, Gabrielle, Ahlgren, Nathan A.
Washington, Analysis, Genetic aspects, Growth, Flow cytometry, Polymerase chain reaction, Nucleic acids, Company growth, Nucleic acid isolation, Synechococcus elongatus

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Detection and visualization of ab exopolysaccharide produced by Xylella fastidiosa in vitro and in planta

Article Abstract:

Polyclonal antibodies are raised against a modified xanthan gum polymer that is similar to the Xylella fastidiosa exopolysaccharide (EPS) polymer, which is quantified by using enzyme-linked immunosorbent assay. Immunolocalization microscopy is used for examining the distribution of Xanthomonas fastidiosa EPS in biofilms formed in vitro and in planta.

author: Kirkpatrick, Bruce C., Roper, M. Caroline, Greve, L. Carl, Labavitch, John M.
Pharmaceutical Preparation Manufacturing, Biological products exc. diagnostic, Polyclonal Antibodies, Physiological aspects, Observations, Xanthan gum

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First evidence of division and accumulation of viable but nonculturable pseudomonas fluorescens cells on surfaces subjected to conditions encountered at meat processing premises

Article Abstract:

The research report designed to model the gradual accumulation of culturable and non culturable flora on a surface in order to explore the phenomena that lead to the establishment of such resident flora. This investigation is expected to support the construction of mathematical model.

author: Carpentier, Brigitte, Peneau, Sophie, Chassaing, Danielle
Usage, Mathematical models, Pseudomonas fluorescens

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subjects list: Research, Bacteriology, Bacterial cultures
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