Culture-dependent and culture-independent characterization of microbial assemblages associated with high-temperature petroleum reservoirs

Article Abstract:

Results demonstrate that sulfur-utilizing and methane-producing thermophilic and mesophilic microorganisms are widely found in oil reservoirs. Data indicate that these bacteria are involved in the biogeochemical transformation of carbon, hydrogen, and sulfur.

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Comparative analysis of methane-oxidizing archae and sulfate-reducing bacteria in anoxic marine sediments

Article Abstract:

Lipid analysis of marine methane seep sediments shows that members of the order Desulfosarcinales and ANME-2 consortia are predominant methane-oxidizers but other methane-consuming and sulfate-reducing bacteria are present as well. This supports the hypothesis that anaerobic oxidation of methane involves a methane-oxidizer and a sulfate-reducing partner.

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Development and field application of a quantitative method for examining natural assemblages of protists with oligonucleotide probes

Article Abstract:

A fluorescent in situ hybridization technique using rRNA-targeted oligonucleotide probes is useful in the enumeration of the eukaryotic protists in environmental water samples and cultures. The counts of protists by this method are similar to those determined by staining methods using acridine orange and 4',-6-diamidino-2-phenylindole. However, the oligonucleotide probes method gives a higher total nanoplankton count. This is probably due to the inability of the staining method to detect small nanoplanktonic cells.

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