Crystal structures of T. brucei MRP1/MRP2 guide-RNA binding complex reveal RNA matchmaking mechanism

Article Abstract:

The structures of Trypanosoma brucei apoMRP1/MRP2 complex and an MRP1/MRP2-gRNA complex are determined to understand the mechanism by which this complex performs RNA matchmaking. The gRNA molecule binds to the highly basic [beta] sheet surface of the MRP complex via non-specific, electrostatic contacts while the gRNA stem/loop II base is anchored to the basic surface, stem/loop I which is unfolded and exposed to solvent.

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Structural basis of core promoter recognition in a primitive eukaryote

Article Abstract:

The crystal structures of the apo-initiator binding domain and initiator binding domain-initiator complexes and the c-terminal domain of the initiator binding protein 39 show winged-helix motif and a scaffold similar to ETS-family proteins for initiator binding domains. Data point out that binding of initiator binding protein 39 to the initiator recruits RNA polymerase II and initiates transcription.

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Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P

Article Abstract:

A study reported the structures of the Bacillus megaterium apoCcpA and a CcpA-(HPr-Ser46-P)-DNA complex. These structures reveal that HPr-Ser46-P mediates a novel two-component allosteric DNA binding activation mechanism.

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