Article Abstract:
An OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex is discussed relative to crystal structure and specific recognition of a protein-DNA interface. The structure of a ternary complex has been established to help in understanding of the structural basis for combinatorial control and sequence-specific recognition in regulation of immunoglobulin gene expression. The OCA-B peptide binds in the major groove near the center of the octamer site. Its polypeptide backbone sets up a pair of hydrogen bonds with the adenine base at position 5 of the octamer DNA.
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Article Abstract:
Dinucleotides GT and AG were recognized with the first intron sequencing, and it was suggested that dinucleotides were almost universal, of a common origin. Later exceptions identified a sub-class with AT and AC at the 5' and 3' boundaries on introns. Examples of intron subsets with boundary sequences AT-AC, possibly spliced by the U12 system, are provided. Further study of U12 spliceosomes may suggest the origin of the parts of both types of spliceosomes and their link to group II-type self-splicing introns.
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Article Abstract:
The discovery-compatible profiling and quantitative measurement of short RNA expression from several ordered stages of T-lymphocyte development is described. The total number of miRNA expressed per cell at different stages of development varies over nearly an order of magnitude in parallel with changes in total cellular RNA content suggesting that global miRNA levels are coregulated with the translational capacity of the cell.
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