Article Abstract:
A microtiter plate model, microscopic examinations and a colorimetir 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium-hydroxide (XTT) reduction assay is used to observe the metabolic activity of cryptococci within a biofilm. It is demonstrated that the fungal biofilm formation depends on support surface characteristics and the growth in the biofilm state makes fungal cells less susceptible to potential environmental stress.
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Article Abstract:
Cryptococcus neoformans are normally white but while carrying out studies of the interaction of Dictyostelium discoideum and C. neoformans in a lawn of feeder Klebsiella aerogenes, it was noticed that fungal colonies near the bacterial colonies were brown. This illustrates that a remarkable interaction takes place between a pathogenic fungus and a gram negative bacterium in which the bacterium produces a substrate that promotes fungal melanization.
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Article Abstract:
The metabolism of melamine by Klebsiella terragena was examined to determine its pathway and the effect of added NH4+ on the rates and coverage of melamine metabolism. The study used separate incubations where cells were grown in ammeline or ammelide. Results suggest that K. terragena is a useful inoculant in conjunction with melamine as a fertlizer as a result of lack of NH4+ inhibition of melamine metabolism.
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