Cross-polarized magic-angle spinning 13C nuclear magnetic resonance spectroscopic characterization of soil organic matter relative to culturable bacterial species composition and sustained biological control of pythium root rot

Article Abstract:

The cross-polarized magic-angle spinning 13C nuclear magnetic resonance spectroscopy shows that the energy available in soil organic matter determines the efficiency of bacteria in biological control of pythium root rot. The rate of hydrolysis of fluorescein diacetate declines below the required threshold level for biological control of pythium root rot, during the initial 10 wk after potting. The carbohydrate concentration also decreases. The total microbial mass and activity remain stable. The composition of the culturable bacterial species changes.

author: Hoitink, Harry A.J., Boehm, Michael J., Wu, Tianyun, Stone, Alex G., Kraakman, Bart, Iannotti, Donna A., Wilson, G. Edwin, Madden, Laurence V.
Usage, Nuclear magnetic resonance spectroscopy, Biomass energy, Humus

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Anastomosis formation and nuclear and protoplasmic exchange in arbuscular mycorrhizal fungi

Article Abstract:

Anastomosis between hyphae from individually germinated spores of arbuscular mycorrhizal (AM) fungi have been monitored through use of time-lapse video-enhanced microscopy, both light- and epifluorescence-based. Anastomosis formation and nuclear and protoplasmic exchange were observed when hyphae originated from the same spore and from different spores of the same isolate of three Glomus species. It appears that genetic exchange may take place through intermingling of nuclei in anastomosis formation.

author: Giovannetti, Manuela, Azzolini, Dario, Citernesi, Anna Silvia
Italy, Statistical Data Included, Anastomosis, Surgical anastomosis, Molecular microbiology

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Stimulation of vesicular-arbuscular mycorrhizal fungi by mycotrophic and nonmycotrophic plant root systems

Article Abstract:

Transformed root cultures of three nonmycotrophic and one mycotrophic plant species stimulated germination and hyphal growth of the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus etunicatum in a gel medium. Roots of the mycotrophic species supported continued hyphal exploration after 3 to 4 weeks and encouraged appressoria formation by Glomus etunicatum. The carrot roots gave rise to spatial information perceived by the fungus, but the non mycotrophic roots did not.

author: Schreiner, Paul R., Koide, Roger T.

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subjects list: Research, Analysis, Root rots, Soil fungi
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