Coupling immunomagnetic separation on magnetic beads with matrix-assisted laser desorption ionization-time of flight mass spectrometry for detection of Staphylococcal enterotoxin B

Article Abstract:

A method is developed based on selective immunoaffinity capture enrichment that can be performed in a single-tube experiment for selective isolation of the protein toxin. The application of this method to complex biological matrices is shown by selective detection of Staphylococcal enterotoxin B (SEB) seen in different matrices like the cultivation media of Staphylococcus aureus and raw milk samples.

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Detection of norovirus capsid protein in authentic standards and in stool extracts by matrix-assisted laser desorption ionization and nanospray mass spectrometry

Article Abstract:

The detection of norovirus capsid protein is explored using three common mass spectrometry (MS)-based methods that are scanning of intact proteins, peptide mass fingerprinting, and peptide sequencing. This is the first report on MS-based detection of norovirus, accomplished by using structurally identical, noninfective virus-like particles at clinically relevant concentrations.

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Detection of staphylococcal enterotoxin B via biomolecular interaction analysis mass spectrometry

Article Abstract:

Research has been conducted on Staphylococcus enetrotoxin B. The authors describe the detection of this enetrotoxin carried out via the use of biomolecular interaction analysis mass spectrometry, and discuss the experiments performed with the standard food samles.

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