Cotransformation of Trichoderma harzianum with beta-glucuronidase and green fluorescent protein genes provides a useful tool for monitoring fungal growth and activity in natural soils

Article Abstract:

Results describe construction of a mitotically stable Trichoderma harzianum cotransformant with genes for hygromycin and green fluorescent protein and beta-glucuronidase markers. Data show that the tool detects and monitors specific strains of the bacterium released into the soil and their growth.

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A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost

Article Abstract:

This article describes the development of a culture medium for selective, quantitative reisolation of Trichoderma harzianum from commercial Agaricus bisporus compost. The culture medium containing the antimicrobials such as chloramphenicol, streptomycin, quintozene, and propamocarb selectively allows recovery of T. harzianum in the form of viable conidia and hyphal fragments in compact colonies.

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Saprotrophic and mycoparasitic components of aggressiveness of Trichoderma harzianum groups toward the commercial mushroom Agaricus bisporus

Article Abstract:

Research shows that aggressive isolates of Trichoderma harzianum produce about 7-8-fold higher biomass in compost, containing Agaricus bisporus, than the nonaggressive isolates. Results indicate that both aggressive isolates secrete depolymerases, which attack the cell walls of A. bisporus. Data suggest that aggressiveness is due to competition, antagonism, or parasitism in a saprophytic growth environment.

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