Cotranscriptional Set2 methylation of histone H3 kysine 36 recruits a repressive Rpd3 complex

Article Abstract:

Biochemical, genetic and gene-expression analyze Rdp3 in two different complexes, the smaller complex Rpd3C(S) shares Sin3 and Ume1 with Rpd3C(L) but contains the unique subunits Rco1 and Eaf3. Chromination immunoprecipitation and biochemical experiments indicated that the chromodomain of Eaf3 recruits Rpd3C(S) to nucleosomes methylated by Set2 on histone H3 lysine 36 leading to deacetylation of transcribed regions.

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Global survey of organ and organelle protein expression in mouse: Combined proteomic and transcriptomic profiling

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The subcellular fractionation with exhaustive tandem mass spectrometry-based shotgun sequencing is combined to examine the protein content of four major organellar compartments. Using rigorous statistical filtering and machine-learning methods, the subcellular localization of 3274 of the 4768 proteins identified was determined, including 1503 previously uncharacterized factors.

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Mapping global histone acetylation patterns to gene expression

Article Abstract:

The genome-wide acetylation profiles for eleven lysines is analyzed in the four core histones of Saccharomyces cerevisiae to search for existence of patterns of acetylation and to analyze their relationship to gene activity. These patterns are generated by histone acetytransferases and deacetylases with specificities for different regions affecting common chromatin regions.

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