Correlation between carbon flux through the pentose phosphate pathway and production of the antibiotic methylenomycin in Streptomyces coelicolor A3(2)

Article Abstract:

Streptomyces coelicolor A3(2) grown in a minimal medium produces methylenomycin as its sole secondary metabolite. Carbon metabolism that occurs during this process is investigated using radiorespirometry. It is shown that the pattern of carbon metabolism switches from the Embden-Myerhof-Parnas pathway to the pentose phosphate pathway during the period of slower growth in batch culture and right before entering the stationary phase. These indicate that the generation of NADPH during the latter part of growth supports the biosynthesis of methylenomycin.

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Ectopic expression of the Streptomyces coelicolor whiE genes for polyketide spore pigment synthesis and their interaction with the act genes for actinorhodin biosynthesis

Article Abstract:

The Streptomyces coelicolor whiE-ORFI to whiE-ORFVII genes, expressed in the plasmids or chromosomes, produce mycelial pigments. The whiE-ORF genes encoding polyketide synthase (PKS) produce actinorhodin in act null mutants. The act genes encoding PKS produce spore pigment in the presence of lesions in the whiE-ORF genes. The whiE-ORFI gene encodes a protein necessary for the proper targeting of the spore pigment. The whiE-ORFII-VIII genes participate in the biosynthesis of the spore pigment. The whiE-ORF genes complement the act genes.

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Interaction between primary and secondary metabolism in Streptomyces coelicolor A3(2): role of pyrroline-5-carboxylate dehydrogenase

Article Abstract:

The addition of three hundred proline to the growth medium of the Gram-positive bacterium Streptomyces coelicolor increases the activity of pyrroline-5-carboxylate dehydrogenase up to three hundred fold. The production of undecylprodigiosin was increased by mutations causing elimination of proline catabolic enzymes. The regulation of biosynthesis takes place at the level of gene transcription activation as indicated by the activity of rifampicin.

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