Article Abstract:
The homeostatic machinery involved in pH maintenance of Lactobacillus plantarum was elucidated. When glucose is present as an energy source, and at low external pH, pyruvate was transported via proton motive force. The transport of pyruvate was accompanied by an influx of protons, reducing the internal pH by one unit. However, the high intracellular concentration of pyruvate was subsequently converted to acetoin, which negated the drop in intracellular pH. The conversion of pyruvate to acetoin is a mechanism for pH homeostasis and detoxification.
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Article Abstract:
Research was conducted to characterize the phenotypic transformations in Nis (super r) Clostridium botulinum 169B. The thermal resistance, membrane fatty acids and germination the mutant were compared with those of the wild-type strain. The nisin-induced increase in the germination rate of wild-type spores took place only in the presence of a germinant which indicates that nisin can be classified as a progerminant, a molecule that stimulates germination in the presence of a germinant.
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Article Abstract:
Research was conducted to examine the combined effects of pH, temperature, protein, lipid and carbohydrate on Clostridium botulinum time-to-toxicity using three modeling methods. Results provide strong evidence that higher concentrations of macromolecules lessen the probability of toxin formation by C. botulinum. Findings also demonstrate that lower pHs and temperatures reduce the chance that C. botulinum will form toxin within the time frame studied.
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