Lactococcus lactis as a cell factory for high-level diacetyl production

Article Abstract:

Research reveals that bacteria can be engineered to produce diacetyl from sugars. Data indicate that about 80% of carbon flux can be rerouted via alpha-acetolactate towards the production of diacetyl by combining the process to NADH oxidase overproduction.

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Increased production of folate by metabolic engineering of Lactococcus lactis

Article Abstract:

Research has been conducted on Lactococcus lactis which synthesizes folate. The authors have identified genes involved in folate biosynthesis in a folate gene cluster of L. lactis, and they report that cloning and overexpression of these genes decrease folate production.

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Controlled modulation of folate polyglutamyl tail length by metabolic engineering of lactococcus lactis

Article Abstract:

The targeted engineering of the folate polyglutamyl tail length by the controlled expression of homologous genes involved in folate biosynthesis and heterologous genes encoding glutamyl hydrolase is described. The engineering studies clearly establish the role of the polyglutamyl tail length in intracellular retention of the folate produced.

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