Contribution of phenazine antibiotic biosynthesis to the ecological competence of fluorescent pseudomonads in soil habitats

Article Abstract:

The production of phenazine antibiotics by Pseudomonas fluorescens 2-79 and P. aureofaciens 30-84 was found to be essential in ensuring their ecological competence by allowing them to compete with resident microflora. Mutant strains unable to synthesize phenazine antibiotics (Phz-) were found to have smaller populations and lower survival rates than the corresponding Phz+ strains. Restoring the phenazine production capacity restored colonization ability and survival rates to levels similar to those of the parent strains.

author: Weller, David M., Thomashow, Linda S., Mazzola, Mark, Cook, R. James, Pierson, L.S., III
Pseudomonas

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Quantification of a 2,4-diacetylphloroglucinol produced by fluorescent Pseudomonas spp. in vitro and in the rhizosphere of wheat

Article Abstract:

Wheat treated with the rifampin-resistant derivative of Pseudomonas fluorescens Q2-87 and grown in raw soils was utilized to produce 2,4-diacetylphloroglucinol (Phl) in a natural rhizosphere environment. The synthetic method produced Phl from soil which can be readily extracted in yields that are greater than conventional methods. Furthermore, the Phl-overproducing strain Q2-87(pPHL5122) generated Phl that is six or seven times higher than the Q287 rifampin-resistant strain.

author: Weller, David M., Thomashow, Linda S., Bonsall, Robert F.
Methods, Analysis, Usage, Escherichia coli, Microbiological synthesis, Microbial biotechnology, Pseudomonas fluorescens, Pharmaceutical microbiology

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Changes in populations of rhizosphere bacteria associated with take-all disease of wheat

Article Abstract:

Chryseobacterium and phlD+ pseudomonads can suppress Gaeumannomyces graminis var. tritici, which causes take-all disease of wheat. These bacteria are more abundant on diseased wheat plants than on healthy plants.

author: Weller, David M., Gardener, Brian B. McSpadden
Fungi, Phytopathogenic, Phytopathogenic fungi

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subjects list: Research, Soil microbiology
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