Contribution of folate biosynthesis to Ralstonia solanacearum proliferation in intercellular spaces

Article Abstract:

The vigorous proliferation of Ralstonia solancearum OE1-1 in host intercellular spaces after the invasion of host plants, which is necessary for the virulence of this bacterium, is studied. It is concluded that folate biosynthesis contributes to the vigorous proliferation of bacteria in intercellular spaces and leads to systemic infectivity resulting in virulence.

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Implications of amino acid substitutions in GyrA at position 83 in terms of oxolinic acid resistance in field isolates of Burkholderia glumae, a causal agent of bacterial seedling rot and grain rot of rice

Article Abstract:

The mechanisms of resistance to oxolinic acid (OA) in Burkholderia glumae field isolates are investigated. The results show that an amino acid substitution at position 83 of GyrA is implicated in OA resistance in field isolates, because among various OA-resistant bacteria only those with a substitution in GyrA might retain the ability to survive on rice plants.

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Construction of chimeric catechol 2,3-dioxygenase exhibiting improved activity against the suicide inhibitor 4-methycatechol

Article Abstract:

A study using the cassette PCR technique to isolate a C23O exhibiting improved activity against 4-mthylcatechol is presented. Cassette PCR is a PCR method that allows a variety of chimeric genes to be isolated without using cloned genes.

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