Contraselectable streptomycin susceptibility determinant for genetic manipulation and analysis of Helicobacter pylori

Article Abstract:

A two-gene cassette that can be selected both, against, because of a Campylobacter jejuni rpsL gene and for, due to an erm gene (erythromycin resistance) is presented. The desired Erm (super s) STR (super r) were acquired at a range of frequencies from 17 to 96% among STr (super r) transformants, with the Erm (super s) yield apparently depending on the strain background and genome location of the targeted locus.

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Atypical helicobacter canadensis strains associated with swines

Article Abstract:

In The Netherlands and Denmark forty-two Helicobacter isolates were isolated from swine feces and all twelve isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis (99% similarity). Amplified fragment length polymorphism genomic typing showed that isolates recovered from swine feces were genetically distinct from H. canadensis strains obtained from humans and geese.

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Direct quantification of Campylobacter jejuni and Campylobacter lanienae in feces of cattle by real-time quantitative PCR

Article Abstract:

Campylobacter jejuni and Campylobacter lanienae in feces of cattle can be directly quantified using nested real-time quantitative (RTQ)-PCR method. The effectiveness of the RTQ-PCR method in quantifying Campylobacter jejuni and Campylobacter lanienae is proved using SYBR green.

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