Construction of improved temperature-sensitive and mobilization vectors and their use for constructing mutations in the adhesin-encoding acm gene of poorly transformable clinical Enterococuss faecium strains

Article Abstract:

A study has been conducted on the problems faced during transformation of clinical isolates of the emerging nosocomial pathogen Enterococcus faecium, which has been hindered by lack of efficient tools. It is observed that the interruption of the acm gene of a nonelectroporable E. faecium wound isolate has been successful.

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Bacterial toxin-antitoxin gene system as containment control in yeast cells

Article Abstract:

Researchers show that the relE and relB genes from E. coli can function in eukaryotic cells, in this case yeast. The relE gene produces a protein that can kill cells and the relB gene produces a protein that counteracts the relE protein. These genes could be used to control genetically modified microorganisms.

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Enhanced iron uptake of Saccharomyces cerevisiae by heterologous expression of a tadpole ferritin gene

Article Abstract:

Researchers describe genetically engineering Saccaromyces cerevisiae with tadpole ferritin. Ferritin is a large macromolecule that can store up to 4,500 iron atoms. This genetically engineered form of yeast could be used as an iron supplement in cattle.

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