Construction of engineered bifunctional enzymes and their overproduction in Aspergillus niger for improved enzymatic tools to degrade agricultural by-products

Article Abstract:

Two chimeric enzymes, FLX and FLXLC, are constructed and successfully overproduced in Aspergillus niger. The results have shown that the fusion of naturally free cell wall hydrolases and an A. niger-derived carbohydrate-binding module (CBM) onto bifunctional enzymes enables the increase of the synergistic effect on the degradation of complex substrates.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Design and production in Aspergillus niger of a chimeric protein associating a fungal feruloyl esterase and a clostridial dockerin domain

Article Abstract:

A chimeric enzyme associating feruloyl esterase A from Aspergillus Niger and dockerin from Clostridium thermocellum is produced. The conclusion states that translational fusion to glucoamylase improved the secretion efficiency of a himeric Doc-FAEA protein and allowed production of the first functional fungal enzyme joined to a bacterial dockerin.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Gene overexpression and biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger

Article Abstract:

The study describes the gene overexpression and the biochemical characterization of the biotechnologically relevant chlorogenic acid hydrolase from Aspergillus niger. The substance is found as a by-product in apple mare and coffee pulp.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: