Article Abstract:
Reverse transcription-polymerase chain reaction techniques were used to clone the genes encoding the variable regions of a monoclonal anti-Bacillus cereus spore IgG. A fusion protein gene was constructed and joined to a strep tag sequence to create a bifunctional single-chain antibody gene. Although similar spore binding behaviors were displayed by the two antibodies, cross-reactions with the spores of B. subtilis and Clostridium perfringens were observed from the single-chain antibody absent in the native monoclonal antibody.
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Article Abstract:
Unique restriction enzyme sites were introduced by amplifying a truncated streptavidin gene by polymerase chain reaction. The amplified sites were linked with the gene of single-chain anti-Bacillus cereus spore antibody to create a fusion protein gene. Identical antigen specificities were exhibited by the native and recombinant monoclonal antibodies. Immobilization of streptavidin-conjugated antibody and its spore binding ability were also described.
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Article Abstract:
The production and characterization of specific antibodies against nonhemolytic enterotoxin (Nhe) subunits NheA, NheB and NheC are described to improve the detection of Bacillus cereus Nhe complex. The results have shown that strains carrying the nhe genes usually express the complete set of the three components, including NheC, but the amount of toxin produced varies considerably between the different strains.
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