Article Abstract:
Two recombinant pathways that allow growth on ortho- and para-substituted chlorobiphenyls as a single source of carbon have been generated following the cloning and expression of the aromatic ring dehalogenation genes in biphenyl-growing, polychlorinated biphenyl-cometabolizing Comamonas testosteroni VP44. The variants were synthesized by transformation of strain VP44 with plasmids containing specific genes for dehalogenation of chlorobenzoates. Mineralization of para-chlorobiphenyls was confirmed by cell protein yield corresponding to complete oxidation of the two biphenyl rings.
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Article Abstract:
A new class of oxygenolytic ortho-dehalogenation (ohb) genes has been cloned from 2-chlorobenzoate- and 2,4-dichlorobenzoate-degrading Pseudomonas aeruginosa 142. Two clones, DHFalphaF'(pOD22) and DHFalphaF'(pOD33), converted 2-chlorobenzoate to catechol and 2,4-dichlorobenzoate and 2,5-dichlorobenzoate to 4-chlorocatechol. The ohb DNA region is flanked by 14-bp imperfect inverted repeats at positions 56 to 69 and 5984 to 5997.
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Article Abstract:
Burkholderia xenovarans strain LB400, which possesses the biphenyl pathway, was engineered to contain the oxygenolytic ortho dehalogenation operon, allowing it to grow on 2-chlorobenzoate and to completely mineralize 2-chlorobiphenyl. Inoculation at different cell densities did not affect the extent of polychlorinated biphenyl biodegradation.
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