Article Abstract:
A study was conducted to determine the characterization of the genes and enzymes responsible for the conversion of glycerol to 1,3-propanediol (PD) operon. Enhancement of the utility of the 1,3-PD genes was analyzed through their rearrangement into a form that would be adaptable to expression in different hosts under any desired regulation. The study revealed that the 1,3-PD operon was designed so that it can be readily altered for expression in other prokaryotic hosts and is therefore helpful in metabolic engineering of 1,3-PD pathways from glycerol and other substrates.
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Article Abstract:
The expression of the cel operon in Escherichia coli, Salmonella typhimurium and Pseudomonas aeroginosa was examined in different temperature regimes. In all the organisms, the cel operon was expressed at elevated temperatures of 48 to 54 degrees celcius. No expression was detected at the mesophilic temperatures. The results show that the expression of the cel operon is regulated by temperature at the transcriptional level. The decryptification of the cel operon may be achieved by temperature-altered DNA superhelicity.
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Article Abstract:
Plasmid pOS25 was constructed to investigate the regulation of the isopropylbenzene (ipb) catabolism operon. The plasmid contains the regulatory elements of the ipb operon, namely ipbR and ipbo/p. Inducers of the ipb operon were found to be hydrophobic compounds and were also observed to include monoalkylbenzenes, substituted benzenes and toluenes, alkanes and cycloalkanes, chlorinated solvents and naphthalenes.
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