Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg2+-contaminated environments

Article Abstract:

Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein substantially increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent characteristics for bioremediation of Hg2+-contaminated locations.

author: Wilson, David B., Chen, Shaolin
Methods, Escherichia coli, Bioremediation

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Cloning, expression, and characterization of cadmium and manganese uptake genes from Lactobacillus plantarum

Article Abstract:

Research conducted on the cloning of cadmium and manganese uptake gene from Lactobacillus plantarum is analyzed. Increase in cadmium uptake and the regulation of its expression are examined.

author: Wilson, David B., Chen, Shaolin, Hao, Zhiqi
Cloning, Gene expression

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Characterization of cadmium uptake in Lactobacillus plantarum and isolation of cadmium and manganese uptake mutants

Article Abstract:

Research conducted on the chemical mutagenesis identification of two cadmium uptake systems is discussed. Experiments to isolate manganese dependent mutant strains are included.

author: Wilson, David B., Hao, Zhiqi, Reiske, Heinz R.
Gene mutations, Gene mutation

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subjects list: Usage, Research, United States, Manganese, Manganese (Metal), Molecular biology, Cadmium
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