Article Abstract:
Saccharomyces cerevisiae conditional mutants with respect to the DNA primases genes, pri1 andpri2, exhibit increased spontaneous mutation and intrachromosomal recombinationrates. This is attributed to the generation of sticky ends resulting from defective replication in the mutants. Sporulation was also compromised in the mutants with unsporulated cells containing one nucleus. This suggests that the mutations have adverse effects on meiosis. Further studies also revealed that the small primase subunit determines enzyme activity while the large subunit mediates interaction between the DNA polymerase alpha and the small subunit.
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Article Abstract:
Research done on the mutatorS (mutS) of Escherichia coli has contributed greatly in explaining tumorigenesis. Siegel and Bryson showed in 1967 that most of the mutations caused by mutS were temporary because the majority could be reverted by 2-aminopurine and ethylmethane sulfate. In 1972, Cox, Degnen and Scheppe reported that mutS cause AT -> GC and GC->AT transitions and that these mutations were very similar to the wild type. No other mutator gene in bacteria or yeast had a similar impact in science. What are missing at the moment are studies on mutagenesis, fitness, selection and drift.
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Article Abstract:
A study was conducted to analyze mutS/hexA homologs from Saccharomyces cerevisiae as mismatch repair proteins. Amplification and cloning of these homologs was done through degenerate oligonucleotide primers based on E coli MutS protein regions and its homologs in polymerase chain reaction. A pair of DNA sequences were then amplified and used to clone genes from a yeast genomic library. Results show that the MutS homolog genes functioned in mitochondrial and nuclear DNA repair.
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