Article Abstract:
The three-dimensional spatial relationships exists between immunostained oocysts and the acridine orange-stained soil matrix material. Oocysts are suspended between soil particles, rather than being attached to them, as revealed by the hydrated, pyrophosphate-dispersed soil preparation. Distinct sporozoites are found in DIC imaging in viable oocysts with the most typical DAPI staining pattern. Computer-assisted laser scanning microscope and color video microscopy are effective techniques for examining Cryptosporidium parvum oocysts.
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Article Abstract:
The capacity of dye permeability assay to identify Cryptosporidium parvum oocysts was examined by comparing the test to an in vitro excystation assay. The assays were used on sodium hypochlorite-permeabilized formaldehyde-fixed and untreated oocysts. Results show that dye permeability viability testing is not based on metabolic activity potential but on oocyst wall permeability. Results also verify the use of dye permeability assay in predicting oocyst infectivity.
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Article Abstract:
Research was conducted to examine the effect of pretreatment and experimental conditions on the hydrophobicity and electrophoretic mobility of Cryptosporidium parvum oocysts. A further objective was to measure the effects of solution ionic strength on the hydrophobicity of polystyrene beads. Results indicate that oocysts purified using the EAPS method displayed an electrophoretic mobility that strongly depended on pH.
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