Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle

Article Abstract:

Components of an Skp1-cullin-F-box (SCF) ubiquitin ligase are discussed relative to their regulation of the centrosome duplication cycle and their localization to the centrosome. Candidate centrosomal F-box proteins have been identified. There is indication that distinct SCF complexes may direct proteolysis of factors that mediate steps in the the centrosome cycle. It has been shown that in mammalian cells the Skp1 protein and the cullin Cul1 are localized to interphase and mitotic centrosomes and to the cytoplasm and nucleus.

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Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor

Article Abstract:

A demonstration that APC/C inhibitor Emi1 tightly binds both the APC/C and its Cdh1 activator, binds to the D-box receptor site on the APC/[C.sup.Cdh1], and competes with APC/C substrates for D-box binding. Emi1 itself contains a conserved C-terminal D-box, which provides APC/C-binding affinity, and a conserved zinc-binding region (ZBR), which antagonizes APC/C E3 ligase activity independent of tight APC binding.

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Emi1 regulates the anaphase-promoting complex by a different mechanism than Mad2 proteins

Article Abstract:

Emi1 has been found to regulate the anaphase-promoting complex by a mechanism different from Mad2 proteins. Emi1 bonds the substrate binding region of Cdc20 and prevents substrate binding to the anaphase-promoting complex/cyclosome.

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