Article Abstract:
Protein components and complexes were studied to identify their effects on DNA replication. Living cells of Saccharomyces cerevisiae were subjected to formaldehyde treatment to cross-link protein-DNA and protein-protein complexes and to identify protein components. Results show that origins of chromatin structure of DNA replication cause a reaction between prereplicative complex in G1 phase cells and also between postreplicative complex in S, G2 and M phase cells. MCM proteins interact with origin recognition complex Cdc45p which plays an important role in replication.
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Article Abstract:
Recombinant forms of the Origin Recognition Complex (ORC) protein prepared with the use of the Baculovirus-infected SF9 cells were used to study the role of ORC in ATP binding and hydrolysis. The results revealed that the Orc1 and Orc5 subunits of the ORC bind ATP. Orc1 was also found to take part in the hydrolysis of ATP. The data showed that the occupancy of the Orc1p ATP-binding site has a profound effect on ORC function and that ATP hydrolysis by Orc1p can drive transitions between different functional states of ORC.
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Article Abstract:
The origin recognition complex 1 protein (Orc1p) of Saccharomyces cervisiae is structurally similar to Cdc6p, Cdc18p, and Sir3p. The yeast proteins Cdc6p and Cdc18p control DNA replication. The region of Orc1p that are similar to Cdc6p probably signals the initiation and completion of transcription. Sir3p controls transcription silencing of the mating-type loci. The N-terminal domain regions, that bind to histones and the regions required for protein localization, are similar in Orc1p and Sir3p.
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