Article Abstract:
Ground anaerobic granular sludge was mixed with Rhine river sediment in a proportion of 1:3 and packed in a fixed bed column to test its ability to reduce tetrachloroethene (PCE). The results showed that in the presence of 1 millimolar lactate, 9 micromoles of PCE was dechlorinated to ethene, which was further reduced to ethane. The conversion was nearly total, at 95% to 98%, withno chlorinated compounds remaining. The reductive dechlorination occured at both 20 and 10 degrees celsius. These results indicate that reductive dechlorination may be used during bioremediation to remove PCE.
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Article Abstract:
The growth kinetics of Pseudomonas sp. B13 on 3-chlorobenzoate (3CB) and acetate was investigated under conditions of minimum substrate concentration (Smin) in a recycling fermentor. Smin was different for batch, chemostat and recycling-fermentor cultures. There was a wide variation in the kinetic parameters for the three experimental conditions. Maintenance energy requirements for cells grown in acetate, 3CB or a mixture of both substrates were dependent on the kinetics of substrate transformation.
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Article Abstract:
Pseudomonas sp. strain B13 has two different transformation systems for 3-chlorobenzoate (3CB). One operates at concentrations over 1 micro mole and has a threshold concentration of 0.50 + - 0.11 micro moles. The other operates at concentrations below 1 micro mole of 3CB and has no threshold concentrations. Transformation kinetics are unaffected by the presence or uptake of acetate and there is no observable induction of residual substrate concentration.
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