Compartmentalized expression of the alpha- and gamma-subunits of the acetylcholine receptor in recently fused myofibers

Article Abstract:

The expression of acetylcholine receptor (AChR) genes during neuromuscular junction (NMJ) formation in mouse diaphragm muscle was studied using an analysis of the distribution of mRNAs for the alpha- and gamma-subunits of the muscle nicotinic AChR. Results revealed that by as early as 13.5 days, during which the first NMJs were formed, nonhomogenous distribution was assummed by these mRNAs. During the next days, extrajunctional mRNAs began disappearing as contrast of synaptic domains increased. By the the first postnatal week was completed, gamma-subunit mRNAs were already undetectable. It was suggested that AChR gene and gamma-subunit gene expression is compartmentalized following the formation of NMJs.

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Modifications of gene expression in myotonic murine skeletal muscle are associated with abnormal expression of myogenic regulatory factors

Article Abstract:

A study has been conducted of the transcripts for the myogenic regulatory factors and the levels of mRNA transcripts which encode the myosin heavy chain isoforms (MyHC) in ADR ('arrested development of righting') and CRP ('cramp') mouse mutants. Results suggest that myotonic muscle pathology may be related to abnormal expression of some myogenic factors. Specific factors appear to be linked to expression of individual MyHC genes. A form of myotonia involving chloride channel dysfunction leads to the induction of mRNA transcripts that code for MyHC isoforms of oxidative fibre types. Both mutant types exhibit qualitatively similar changes in mRNA transcripts.

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Two types of neonatal-to-adult fast myosin heavy chain transitions in rat hindlimb muscle fibers

Article Abstract:

Immunohistochemistry and S1 nuclease mapping were used to review exploration of the function of denervation in the neonatal-to-adult fast myosin heavy chain (MHC) protein, and mRNA transitions in the development of rat fast-twitch muscles. The levels of neonatal MHC mRNA do not fall in muscles denervated at birth, and this mRNA is reexpressed in the denervated muscles of young adult rats.

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