Comparison of quasielastic light scattering and laser diffractometry as nondestructive probes into the structure of Bacillus sphaericus spores produced at different temperatures

Article Abstract:

Experiments are performed to determine the feasibility of using quasielastic light scattering (QLS) and laser diffractometry as non-destructive probes for studying the size of bacterial pores in suspension. The two methods are used in the investigation of Bacillus sphaericus. Results show that QLS can be used in studying the gross conformation and changes that occur during germination or destruction of ellipsoidal spores. LC can only be used in the analysis of spherical particles.

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The introduction into Bacillus sphaericus of the Bacillus thuringinesis subsp. medellin cyt1Ab1 gene results in higher susceptibility of resistant mosquito larva populations to B. sphaericus

Article Abstract:

A study was conducted to introduce the fragment supporting the gene encoding the cytolytic Cyt1Ab1 protein is its flanking sequences into the strains of Bacillus sphaericus. Plasmid DNA was determined and purified from Escherichia coli using the Qiagen plasmid kid. DNA fragments were then subjected to electrophoresis in agarose gels. In addition, the Bradford assay was used to determine the protein concentrations of alkali-solubilized bacterial suspensions and purified crystals.

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Identification of a gene for Cyt1A-like hemolysin from Bacillus thuringiensis subsp. medellin and expression in a crystal-negative B. thuringiensis strain

Article Abstract:

The gene cyt1Ab1, encoding cytolysin from the mosquitocidal strain Bacillus thuringiensis subsp. medellin, is expressed in a crystal-negative strain of B. thuringiensis, that is transformed with the gene. Cyt1Ab1 is similar to the cyt1Aa1 gene of B. thuringiensis subsp. israelensis, but its toxicity is lower than cyt1Aa1. The purified crystals of cyt1Ab1 are as hemolytic as cyt1Aa1 gene and twice as hemolytic as the wild-type strain.

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