Pseudomonas aeruginosa cystic fibrosis clinical isolates produce exotoxin A with altered ADP-ribosyltransferase activity and cytotoxicity

Article Abstract:

Clinical isolates of Pseudomonas aeruginosa from cystic fibrosis infections contain high levels of toxA, responsible for encoding the virulence factor exotoxin A . Data point out that mutations in the enzyme ADP-ribosyltransferase produce less active exotoxin A involved in the cytotoxicity and chronic lung infections of cystic fibrosis patients.

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Comparison of proteins expressed by Pseudomonas aeruginosa strains representing initial and chronic isolates from a cystic fibrosis patient: an analysis by 2-D gel electrophoresis and capillary column liquid chromatography-tandem mass spectrometry

Article Abstract:

A study taking advantage of availability of the completed genome of Pseudomonas aeruginosa and the techniques of two-dimensional gel electrophoresis followed by mass spectrometry to detect variations in protein expression between nonmucoid and mucoid P. aeruginosa strains recovered from a cystic fibrosis (CF) patient was carried out. The study compared strains that represent those from initial and chronic lung infections in CF patients to begin to find out how specific proteins may contribute to the observed phenotypic differences between the strains. Differences identified will be helpful in further studies and in looking at the potential of the proteins as novel drug targets or as vaccine candidates.

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Secreted products of a nonmucoid Pseudomonas aeruginosa strain induce two modes of macrophage killing: external-ATP-dependet, P2Z-receptor-mediated necrosis and ATP-independent, caspase-mediated apoptosis

Article Abstract:

Secreted products of a nonmucoid Pseudomonas aeruginosa strain, strain 808, have been found to bring on two types of macrophage killing. One is external-ATP-dependent, P2Z-receptor-mediated necrosis and the other ATP-independent, caspase-mediated apoptosis. Cyotoxic factors active against macrophages were secreted during the exponential phase of growth in a complex medium. Two secreted redox proteins, azurin and a cytochrome c, induce mast cell apoptosis where there is no exogenous ATP. Researchers are interested in whether secretion of ATP-utilizing enzymes and associated cyotoxic agents is restricted to cystic fibrosis isolate mucoid strains. The nature of the pathway promoting macrophage cell death through the P2Z-receptor-independent pathway is of interest.

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