Comparison of identification systems for classification of bacteria isolated from water and endothilic habitats within the deep subsurface

Article Abstract:

Deep subsurface samples of water and rock were obtained from the tunnel system of the Rainier Mesa at the Nevada Test Site to determine the microbial populations occupying this particular niche. To identify the isolated microorganisms, three identification systems, API-NFT strips, BIOLOG and MIDI, were used. The three systems were able to identify only a small proportion of the total isolates and showed considerable inconsistencies at the species level. The most commonly identified genus was Pseudomonas. In addition, population profiles of the rock samples differed from that of the water sample.

author: Amy, P.S., Haldeman, D.L., Ringelberg, D., Hall, D.H., Russell, C.
Evaluation, Medical testing products, Nevada Test Site, Diagnostic reagents and test kits, Diagnostic reagents

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Enumeration and characterization of bacterial colonists of a submersed aquatic plant, Eurasian watermilfoil (Myriophyllum spicatum L.)

Article Abstract:

A simple classification scheme for the identification of aerobic heterotrophic bacteria colonizing Eurasian watermilfoil was developed. The scheme made use of colony, cell and biochemical characteristics to differentiate the various colonists. Application of the procedure showed that nutrient-poor media was better able to support defined and stable growth of various species. The watermilfoil-associated bacteria were identified as Acinetobacter, Cytophaga, Flavobacterium, Moraxella, Pseudomonas/Alcaligenes, Vibrio/Aeromonas, Micrococcus and enterobacters.

author: Chand, T., Harris, R.F., Andrews, J.H.
Aquatic weeds

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Characterization of an endosymbiont infecting wood ticks, Dermacentor andersoni, as a member of the genus Francisella

Article Abstract:

A study on Dermacantor andersoni reveals that the microorganism is an endosymbiont from the genus Francisella. PCR assay shows that the 16S rDNA gene sequence of the symbiont is 95.4% similar to the sequence of Francisella tularensis subsp tularensis. Almost all of the sample ticks collected in the the Bitterroot Mountains from 1992-1996 were found to be infected with the microorganism.

author: Fischer, Elizabeth R., Schwan, Tom G., Porcella, Stephen F., Niebylski, Mark L., Peacock, Mort G.
Discovery and exploration, Ticks

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subjects list: Research, Identification and classification, Microbiology, Taxonomy (Biology), Microorganisms
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