Comparison of PCR and plaque assay for detection and enumeration of colphage in polluted marine wasters

Article Abstract:

Reverse transcriptase polymerase chain reaction and plaque assay were used to identify enteric viruses in marine waters polluted with sewage and storm runoffs. A minimum probable number method was used to quantify the results of reverse transcriptase polymerase chain reaction. There were discrepancies in the results obtained from both methods. These discrepancies may be due to the presence of nonculturable coliphage, the difference in the sensitivity and specificity of the assays or the inhibition of reverse transcriptase polymerase chain reaction in the marine samples.

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Detection of viral pathogens by reverse transcriptase PCR and of microbial indicators by standard methods in the canals of the Florida Keys

Article Abstract:

A survey was conducted to evaluate the concentration of microbial fecal indicators and the presence of human pathogenic microorganisms, thus, assessing the microbial water quality in canal waters throughout the Florida Keys. The assaying of total coliforms, fecal coliforms and other viruses were done at 17 canal sites and two nearshore water sites. Results suggest that the canals and nearshore waters throughout the Florida Keys were being affected by human fecal material having human enteric viruses through current wastewater treatment techniques such as septic tanks.

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Molecular assays for targeting human and bovine enteric viruses in coastal waters and their application for library-independent source tracking

Article Abstract:

The extent and relative importance of fecal contamination from agricultural activities, anthropogenic activities, and development upstream in coastal reaches of the lower Altamaha River, Georgia, is examined by detecting three groups of host-specific enteric viruses- Human enteroviruses human adenoviruses, and bovine enteroviruses (BEV). The results indicate that the stringent host specificity of enteric viruses makes them good library-independent indicators for identifying sources of water pollution.

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