Detection and characterization of plasmid pJP4 transfer to indigenous soil bacteria

Article Abstract:

Plasmid transfer was detected from an E. coli with the pJP4 plasmid to other bacteria in nonsterile soil. The plasmid codes for mercury resistance and partial 2,4-dichlorophenoxyacetic acid degradation. Most of the bacteria that received the plasmid were Burkholderia or Pseudomonas species.

author: Newby, D.T., Josephson, K.L., Pepper, I.L.
Genetic engineering, Identification and classification

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Comparison of 2,4-dichlorophenoxyacetic acid degradation and plasmid transfer in soil resulting from bioaugmentation with two different pJP4 donors

Article Abstract:

It may be critical to choose the best donor host bacterium when attempting to inoculate contaminated soil with plasmids that can be transmitted to other soil bacteria. This technique is used to introduce a plasmid for biodegradation of toxic compounds into a community of soil bacteria.

author: Newby, D.T., Pepper, I.L., Gentry, T.J.
Biodegradation

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Dual-bioaugmentation strategy to enhance remediation of cocontaminated soil

Article Abstract:

Researchers show that the co-inoculation of soil with two different types of bacteria can remediate soil contaminated by both metals and organic pollutants. Arthrobacter, Bacillus, and Pseudomonas species that were resistant to cadmium were used to protect the organic-degrading bacterium Ralstonia eutropha in the laboratory and in the field.

author: Josephson, K.L., Pepper, I.L., Roane, T.M.
Soils, Bioremediation, Contamination

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subjects list: Research, Plasmids, Soil microbiology
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