Article Abstract:
Recovery of cysts is more efficient than recovery of vegetative forms, regardless of the concentration techniques employed. This was gleaned from a study of the recovery of Naegleria fowleri, in vegetative or cystic form, that had been mixed with the two N. species of N. lovaniensis and N. australiensis, using filtration and centrifugation. Results led to the conclusions that the cytoplasmic membrane of the vegetative form may be very sensitive to adverse conditions during isolation and that there is significant imprecision and inaccuracy in the evaluation of N. concentrations.
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Article Abstract:
Examination of the composition of dissolved DNA in marine environments by differential centrifugation and probing with 16S rRNA-targeted kingdom oligonucleotide probes reveals that 50% of the filterable DNA is free DNA, while the remaining portion contains vital particles and colloids. Viral abundance tests reveal that viral particles accounts for 17 to 30% of the remaining portion. Dissolved DNA undergoes hybridization with universal, eubacterial, and eucaryotic probes, while purified standard viral DNAs hybridize only with the universal probe.
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Article Abstract:
Tests studying the reliability of the centrifugation and filtration techniques used to determine the presence of Legionella in water show that filtration using flat membrane gives the best results. All concentration methods are inaccurate and cannot be used if the Legionella concentration in the water is low. The results obtained were different from those obtained for actual water samples because of the seeded water samples used in the study.
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