Comparative growth rates of various rumen bacteria in clarified rumen fluid from cows and sheep fed different diets

Article Abstract:

The relative growth rates of different species of rumen bacteria in rumen fluid were determined. Stains of Bacteroides ruminicola, Butyrivibrio fibriosolvens, Eubacterium cellulosolvens and Ruminococcus albus among others were grown in sterilized rumen fluid augmented with glucose and bicarbonates as energy source and buffer, respectively. Cysteine and sulfide were also added to the medium as reducing agents. Results showed that glucose and other nitrogen sources such as ammonia, amino acids and peptides stimulated the growth were not the primary growth factors for the bacterial species in the rumen.

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The leucine incorporation method estimates bacterial growth equally well in both oxic and anoxic lake waters

Article Abstract:

Estimating bacterial biomass production by measuring leucine uptake is accurate in anoxic environments, according to researchers who used the method in oxic and anoxic water from three lakes with different nutrient and humic content. Leucine uptake is fast and simple to measure and has supplanted other methods based on the frequency of dividing cells or incorporation of thymidine into DNA.

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Use of the ((super.14)C)leucine incorporation technique to measure bacterial production in river sediments and the epiphyton

Article Abstract:

The ((super.14)C)leucine incorporation technique is discussed. It is used to measure bacterial production in the epiphyton and river sediments. The method can measure bacterial production in a wide range of aquatic habitats, fluvial sediments included, as long as substrate saturation and isotope dilution are fojnd.

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