Marine planktonic archaea take up amino acids

Article Abstract:

Researchers have discovered archaea in a non-extreme environment about 200 meters below the surface in the Mediterranean and Pacific Oceans. The bacteria represented 43% and 14% of the total cells, respectively, and actively took up dissolved amino acids. This indicates that they are capable of competing with other bacteria in non-extreme environments.

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Increase in fluorescence intensity of 16S rRNA in situ hybridization in natural samples treated with chloramphenicol

Article Abstract:

The increase in flourescence intensity of 16S ribosomal RNA in situ hybridization in natural samples treated with chloramphenicol was studied to improve detection of marine prokaryotes with the increase of cellular rRNA content without altering the species composition. Results suggest that chloramphenicol treatment of marine samples can help increase the overall flourescence signal for in situ hybridization.

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Combined microautoradiography-16S rRNA probe technique for determination of radioisotope uptake by specific microbial cell types in situ

Article Abstract:

A novel method using fluorescent in situ hybridization and microautoradiography was developed to examine and determine the function of specific microbial groups. The proposed method, which was named substrate-tracking autoradiographic fluorescent in situ hybridization, involves the use of a triple-labeling technique. This method was observed to have the capacity to calculate in situ nutrient uptake of each member of specific picoplankton groups.

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