Article Abstract:
Expression of the genes coding for Aspergillus nidulans endoxylanase X24 and Aspergillus oryzae alpha-amylase in industrial baker's yeasts was investigated by molecular methods. The results revealed that expression of these enzymes in yeasts which are used for bread making influences the quality of bread. Yeast transformation experiments showed that bread made with transformants expressing both enzymes exhibits increased volume and reduced firmness.
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Article Abstract:
The role of the salt-responsive calcineurin-Crz1p-pathway in mediating tolerance to freezing by industrial baker's yeast was investigated. It was found that the overexpression of CRZ1 in the industrial strain increased both salt and freeze tolerance improving the leavening ability of baker's yeast in high sugar dough suggesting that expression of calcineurin-Crz1p target genes can alleviate the harmful effects of ionic stress during freezing.
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Article Abstract:
Recombinant [beta]-hexosaminidase A (HexA) was produced in the methylotrophic yeast Ogataea minuta to prepare a large amount of HexA for treatment based enzyme replacement therapy (ERT). The problem of antigenicity due to differences in N-glycan structures between mammalian and yeast glycoproteins was resolved by using [alpha]-1,6-mannosyltransferase deficient yeast as the host.
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