Combined approach for characterization of uncultivated magnetotactic bacteria from various aquatic environments

Article Abstract:

The diversity and succession of magnetotactic bacteria (MTB) populations in various microcosms from different aquatic habitats is investigated using a combination of a culture-independent 16S rRNA gene-based approach with extensive cultivation experiments. A novel magnetotactic rod (MHB-1) is detected in a sediment sample from a lake in northern Germany by fluorescence in situ hybridization.

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First genome data uncultured upland soil cluster alpha methanotrophs provide further evidence for a close phylogenetic relationship to Methylocapsa acidiphila B2 and for high-affinity methanotrophy involving particulate methane mononxygenase

Article Abstract:

A study is conducted to gain the first insight into the genome of a USCalpha representative by using a metagenomic approach. Results reveal that both the pmo operon structure and the predicted secondary structure of deduced particulate methane monooxygenase are highly conserved among all methanotrophic bacteria.

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Inactivation of the flagellin gene flaA in magnetospirillum gryphiswaldense results in nonmagnetotactic mutants lacking flagellar filaments

Article Abstract:

A method for knockout mutagenesis by homologous recombination in magnetospirillum gryphiswaldense is reported. Multiple flagellin genes are identified and it's targeted diruption eliminate flagella formation, motility and manetotaxis.

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