Article Abstract:
A colony blotting procedure for the detection and differentiation of botulinal toxin type in and around colonies of Clostridiu botulinum types A, B and E and of toxigenic C. butyricum was developed. The procedure involved toxin transfer to nitrocellulose filters followed by probing using type-specific antitoxins. The initial results showed a high degree of cross-reaction between types A and B antitoxins. These were reduced by adsorption to crude toxin concentrates and vegetative cells of the opposite type bacterium. The procedure is not recommended to replace the standard mouse bioassay.
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Article Abstract:
The consumption of excess levels of arginine by Clostridium botulinum Okra B was studied. Similar patterns of growth but slightly different lysis were observed with basal and high levels of arginine. Arginine, however, was not the major source of energy. The utilization of high level of arginine by C. botulinum led to formation of citrulline and proline. They were also produced but reassimilated in media with low level of arginine. Arginine metabolism, therefore, could provide protection against increased acidity and osmolarity.
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Article Abstract:
The boticin B gene of Clostridium botulinum resides on an 18.8 kb plasmid and encodes a protein of 50 amino acids. Boticin B is a bacteriocin that inhibits various clostridia, including C. botulinum.
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