Clustering of trichothecene-producing Fusarium strains determined from 28S ribosomal DNA sequences

Article Abstract:

The five prime end of the large nuclear ribosomal DNA (rDNA) of the coding region of Fusarium strains were analyzed to determine the genetic relationship of the trichothecene-producing microbe. Sequence analysis of the DNA at the five prime end of the large nuclear rDNA indicated a correlation between trichothecene production and the phylogenetic placement of a large number of Fusarium species. Furthermore, the correlation between trichothecene production and rDNA sequence indicated the need for a revision of the systematics of the Fusarium strains.

author: Logrieco, Antonio, Mule, Giuseppina, Bottalico, Antonio, Stea, Gaetano
Identification and classification, Microbiology, Bacterial genetics, Fusarium, Trichothecenes

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The faeA genes from Asperigillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides

Article Abstract:

A research was conducted to investigate the characterization and cloning of a gene encoding a ferulic acid esterase, faeA, from Aspergillus niger and Aspergillus tubingensis. The faeA gene was induced by growth on wheat arabinoxylan and sugar beet pectin. Polyclonal antibodies were utilized in the analysis of supernatant samples from Aspergillus cultures. Results indicated a lipaselike serine active site in the gene product when there was spacing within the catalytic trade from lipases.

author: Visser, J., Vries, R.P. de, Michelsen, B., Poulsen, C.H., Kroon, P.A., Heuvel, R.H.H. van den, Faulds, C.B., Williamson, G., Hombergh, J.P.T.W. van den
Esterases, Polysaccharides, Complex compounds, Coordination compounds

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Ochratoxin A production and amplified fragment length polymorphism analysis of Aspergillus carbonarius, Aspergillus tubingensis, and Aspergillus niger strains isolated from grapes in Italy

Article Abstract:

A study was conducted to identify 77 black aspergilli isolated from grape berries collected in a 2-year survey in 16 vineyards throughout Italy, using amplified fragment length polymorphisms (AFLPs) and genomic DNA sequences. The results showed that ochratoxin is produced by A. tubingensis and it may be responsible for the ochratoxin contamination of wine in Italy, when it is together with A. carbonarius and A. niger.

author: Logrieco, Antonio, Mule, Giuseppina, Perrone, Giancarlo, Susca, Antonia, Pietri, Amedeo, Battilani, Paola
Italy, Science & research, Grape Vineyards, Grapes, Physiological aspects, Nutritional aspects, Food contamination, Mycotoxins, Properties

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subjects list: Research, Genetic aspects, Aspergillus
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